Control of neuronal cell adhesion on single-walled carbon nanotubes 3D patterns

Carbon nanotubes are emerging substrates for guiding neuronal cell growth. Here we investigate the
influence of multiscale 3D architecture of single-walled carbon nanotubes (SWCNT) on the adhesion of
human neuronal cells (neuroblastoma SHSY5Y). 3D patterns of SWCNT were fabricated by
a templating process to yield a hexagonal array of interconnected SWCNT semicapsules with
controlled multiscale porosity, and integrated between Pt electrodes. Neuronal cells adhered
preferentially to the SWCNT semicapsules with respect to either silicon oxide or disordered networks of
SWCNT. Morphological cell features (size and shape) were evaluated upon application of an electric
field across SWCNT pattern. Cell adhesion is enhanced by an electric field above 1 V cm1, whereas it is
completely depleted at 5 V cm1. This shows the possibility to tune neuronal cell adhesion across
different regimes by means of 3D pattern and voltage-biasing SWCNT