Molecular characterization of a variable tandem repeat sequence determined during RADP analysis among Posidonia oceanica insular and coastline population
Academic Article
Publication Date:
2002
abstract:
Abstract--The seagrass Posidonia oceanica plays a multifunctional role in the coastal area as an important and productive
component of ecosystems in the Mediterranean Sea. We detected by RAPD analysis with two arbitrary primers
genetic differences in P. oceanica collected from several sites in the Southern Mediterranean. By AMOVA analysis
we observed a level of about 20% genetic difference among individuals within a population and 80% among
populations. A common band of 200 bp was found in all the amplified samples. Cloning and sequencing analysis of
this band revealed the presence of a simple tandem repeat sequence (minisatellite) that we called PoTR (Posidonia
oceanica tandem repeat). Finally, the ability of PoTR to detect genetic variability in P. oceanica genome was demonstrated
by the presence of amplification products of different lengths utilizing primers internal to this sequence.
component of ecosystems in the Mediterranean Sea. We detected by RAPD analysis with two arbitrary primers
genetic differences in P. oceanica collected from several sites in the Southern Mediterranean. By AMOVA analysis
we observed a level of about 20% genetic difference among individuals within a population and 80% among
populations. A common band of 200 bp was found in all the amplified samples. Cloning and sequencing analysis of
this band revealed the presence of a simple tandem repeat sequence (minisatellite) that we called PoTR (Posidonia
oceanica tandem repeat). Finally, the ability of PoTR to detect genetic variability in P. oceanica genome was demonstrated
by the presence of amplification products of different lengths utilizing primers internal to this sequence.
Iris type:
01.01 Articolo in rivista
List of contributors:
Romancino, Daniele; DI CARLO, Marta
Published in: